Controls

As in any SIP-experiment, appropriate no-label controls should be set up in parallel to avoid detecting false-positives. Particularly with the growing use of high-throughput sequencing and statistical models to detect labelled OTUs the need to include more no-label controls in the experiment in order to correctly detect labelled phylotypes has been growing. The exact number of no-label controls will depend on the exact statistical method used to analyse the data, but also on the type of SIP being performed since DNA-SIP is more prone to detecting false positives than RNA-SIP because of the effect of the G+C-content  bias (see \ref{316470} for more details). Ideally, every labelled sample will have its parallel no-label control.